A review of research on the rapid detection of foodborne pathogenic bacteria by duplex PCR
食源性致病菌二重PCR法快速检测研究综述

摘要

随着社会对食品安全问题关注度的不断提升，快速检测食源性致病菌成为预防和控制食源性疾病的重要手段。PCR检测法作为分子生物学和医学诊断中常用的检测手段，在食源性致病菌检测中同样具有重要作用。本文即在此背景下展开综述研究，通过分析相关概念内容，分别对食源性病原菌检测技术、食源性致病菌PCR检测技术、食源性致病菌PCR检测标准以及食源性致病菌二重PCR法快速检测等相关文献进行总结分析，以此为广大食品安全检测人员提供一定的技术参考。

Abstract

With the escalating public focus on food safety, the expedited detection of foodborne pathogenic bacteria has emerged as a pivotal strategy for the prevention and management of foodborne diseases. The PCR detection method, extensively utilized in molecular biology and medical diagnostics, is also critically important for the identification of foodborne pathogens. This scholarly article presents a comprehensive overview within this context, analyzing pertinent concepts and synthesizing research on foodborne pathogen detection technologies, PCR detection methodologies, PCR detection standards, and the rapid identification of foodborne pathogens via duplex PCR. It is intended to serve as a technical reference for professionals engaged in food safety testing.